SULFHYDRYL GROUPS AND GRAM STAIN

Ribonucleates and the Gram stain.

Salton (J. Gen. Microbiol. 30:223, 1963) summarized the evidence which demonstrates that the gram-positive cell is able to retain the crystal violet after iodine treatment because of the characteristic nature of its cell wall. This evidence is highly convincing, yet it does not explain the experiments of Henry and Stacy (Proc. Roy. Soc. London Ser. B 133:391, 1946) or of Bartholomew and Umbreit...

The Gram stain.

Mechanism of gram stain reversal.

This paper deals with an investigation of the effect of these reactions on bacteria. Not only the common staining behavior but also certain other similarities (Fischer et al., 1949; Fischer, 1951) between intact wool and gram negative bacteria, medium degraded wool and gram variable bacteria, as well as between highly degraded wool and gram positive bacteria suggested that reversal of gram stai...

Sulfhydryl Groups in Proteins

Egg albumin in the native, unaltered state, does not give tests characteristic of sulfhydryl groups. When, however, this protein is treated in any one of several ways, such as by heat (8, 9, 17), ultraviolet irradiation (lo), shaking (lo), or by solution in urea or other amides (7), free sulfhydryl groups make their appearance. The amount of free -SH groups appearing in egg albumin through the ...

Gram-Positive Diplococci in a Cerebrospinal Fluid Gram Stain

which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact [email protected].